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  • Writer's pictureJeremy Wright

Pasteurization vs. Sterilization in Mushroom Cultivation: A Comparative Guide

Mushroom cultivation involves creating a controlled environment to encourage the growth of desired mushroom mycelium while minimizing the development of competing microorganisms. Two common techniques used to achieve this are pasteurization and sterilization. Let's compare and contrast these practices to understand their benefits, limitations, and when to use each method.


What is it? Pasteurization involves heating a substrate to temperatures lower than those used in sterilization. The goal is to reduce the population of competing microorganisms while preserving some beneficial microorganisms, allowing mushroom mycelium to thrive.


  1. Temperature: Pasteurization is typically done at temperatures between 140°F to 160°F (60°C to 71°C).

  2. Time: The substrate is exposed to these temperatures for around 60 to 90 minutes.

  3. Effect: Pasteurization kills many harmful contaminants while still retaining some microorganisms that might be beneficial for mycelium growth.


  • Retains beneficial microorganisms that help protect against contamination.

  • Ideal for species sensitive to high heat.

  • Reduced risk of overheating or damaging the substrate.


  • Some contaminants may survive pasteurization.

  • Shorter shelf life compared to fully sterilized substrates.

  • Not suitable for all mushroom species.

When to Use Pasteurization:

  • Species Preference: Species like Oyster mushrooms and Wine Cap mushrooms tend to do well with pasteurized substrates.

  • Home Cultivation: Ideal for hobbyists and beginners due to its relative simplicity and reduced risk of overheating.


What is it? Sterilization involves subjecting the substrate to high temperatures to completely eliminate all microorganisms, including beneficial ones. This creates a clean slate for mushroom mycelium growth but requires careful measures to prevent contamination during inoculation.


  1. Temperature: Sterilization is typically done at temperatures around 250°F to 270°F (121°C to 132°C).

  2. Time: The substrate needs to be exposed to these high temperatures for an extended period, often around 60 to 90 minutes.

  3. Effect: Sterilization eradicates nearly all microorganisms, providing a pristine environment for mushroom mycelium to colonize.


  • Drastically reduces contamination risks.

  • Longer shelf life of sterilized substrates.

  • Can be used with a broader range of mushroom species.


  • Can damage or alter the nutritional content of the substrate.

  • Requires more careful precautions to avoid contamination during inoculation.

  • Some mushroom species may struggle to colonize sterilized substrates.

When to Use Sterilization:

  • Commercial Cultivation: Used by professional cultivators to ensure consistent yields and quality.

  • Substrate Type: Suitable for substrates with high nutrient content, such as grains and supplemented sawdust.

  • Contamination Prone Species: Some species, like Shiitake mushrooms, benefit from sterile conditions to outcompete contaminants.


The choice between pasteurization and sterilization depends on factors like the mushroom species you're cultivating, your level of experience, and the substrate you're using. While pasteurization maintains some beneficial microorganisms and is more forgiving, sterilization provides a clean slate and reduces contamination risks. Both methods have their place in mushroom cultivation, and understanding when to use each technique is key to successful and bountiful harvests.

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