top of page
  • Writer's pictureJeremy Wright

Fungi Liquid Culture 101: A Beginner's Guide

Welcome to our beginner's guide to fungi liquid culture! Whether you're an aspiring mycologist, a hobbyist, or a curious learner, this blog post will provide you with all the essential information you need to understand and start your own fungi liquid cultures.

What is Fungi Liquid Culture?

Fungi liquid culture, often referred to as LC, is a method used to grow fungi in a liquid medium. It's a versatile and efficient technique that allows for the rapid expansion of fungal mycelium, making it a popular choice among mushroom cultivators.

Advantages of Liquid Culture:

  1. Rapid Growth: Fungi can grow much faster in liquid culture compared to solid substrates like agar.

  2. Easy Inoculation: Liquid cultures offer a convenient way to inoculate substrates for spawn production or fruiting.

  3. High Yield: Liquid cultures can produce a large volume of mycelium in a relatively short time, leading to higher yields of mushrooms.

Basic Equipment Needed:

  1. Sterilized Jars or Bottles: Glass jars or bottles with tight-fitting lids are commonly used containers for liquid cultures.

  2. Liquid Culture Medium: Typically, a nutrient-rich solution is used as the growth medium. Common recipes include malt extract agar (MEA) or potato dextrose broth (PDB).

  3. Inoculation Tools: Sterile syringes or inoculation loops are used to transfer fungal spores or mycelium into the liquid culture medium.

  4. Sterilization Equipment: A pressure cooker or autoclave is essential for sterilizing both the liquid medium and the equipment used in the process.

  5. Incubation Setup: A warm and dark environment is needed to encourage fungal growth. An incubator or a warm closet can serve this purpose.

Steps to Prepare Fungi Liquid Culture:

  1. Prepare the Liquid Medium: Follow a recipe to prepare your chosen liquid medium. Sterilize it using a pressure cooker or autoclave to eliminate any contaminants.

  2. Inoculate the Medium: Using sterile techniques, transfer a small amount of fungal spores or mycelium into the liquid medium. Seal the container tightly.

  3. Incubate the Culture: Place the inoculated jars or bottles in a warm and dark environment. Monitor the cultures regularly for signs of growth.

  4. Maintain and Subculture: As the mycelium grows, you can use portions of the liquid culture to inoculate new batches or substrates. Regularly check for contamination and transfer healthy cultures to fresh medium.

Tips for Success:

  • Practice Sterile Technique: Contamination is a common issue in fungi cultivation. Practice good sterile technique to minimize the risk of contamination.

  • Monitor Temperature: Maintain a consistent temperature within the incubation setup to promote optimal fungal growth.

  • Use Quality Ingredients: Ensure that you're using high-quality ingredients for your liquid culture medium to provide essential nutrients for fungal growth.

  • Keep Records: Keep detailed records of your liquid culture experiments, including the species used, medium composition, and growth observations. This will help you learn and improve your techniques over time.


Fungi liquid culture is a valuable technique for anyone interested in growing mushrooms or studying fungal biology. With the right equipment, sterile technique, and proper maintenance, you can harness the power of liquid culture to produce healthy mycelium for a variety of applications. So, roll up your sleeves, gather your supplies, and embark on your fungi liquid culture journey today!

Happy Cultivating! 🍄

75 views0 comments

Recent Posts

See All


Post: Blog2_Post
bottom of page