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  • Writer's pictureJeremy Wright

Correct Storage Temperature for Liquid Culture

The correct storage temperature for liquid culture in mushroom cultivation is typically around 40°F to 50°F (4.4°C to 10°C). This temperature range is commonly recommended to slow down the metabolic activity of the mycelium and preserve the liquid culture for an extended period while minimizing the risk of contamination. Here's why this temperature range is ideal for storing liquid culture:

  1. Slowed Growth: Lower temperatures slow down the metabolic processes of the mycelium in the liquid culture. This reduced activity helps maintain the viability of the mycelium over time.

  2. Contamination Prevention: While the lower temperature doesn't guarantee complete prevention of contamination, it creates an environment that is less favorable for the growth of contaminants. This helps extend the storage life of the liquid culture.

  3. Longevity: Storing liquid culture at cooler temperatures can extend its viability for several months, allowing you to keep a stock of healthy mycelium for future projects.

It's important to note that even with the recommended storage temperature, periodic checks and transfers to fresh culture might be necessary to ensure the health and viability of the mycelium. Additionally, maintaining proper aseptic techniques during storage and transfers is crucial to prevent contamination and maintain the integrity of the liquid culture. Remember that different mushroom species and strains may have varying tolerances and requirements, so always refer to the specific guidelines provided by experts or reputable sources for the optimal storage conditions of your chosen mushroom liquid culture.


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